Dr. Bruno Torcal Embeita
Other FPI grant
- obnru [dot] h [at] gmail [dot] com
- 943 01 8841
- Research Overview
Studying the cis-trans isomerization fo the cromophore of the IrisFP fluoroprotein, within the research line "Optical properties and excited-state dynamics of photo-active proteins".
Physics of Nanostructures and Advanced Materials --->
PHOTOPHYSICS OF FLUORESCENT PROTEINS
Brief description of the project:
- Resolve the structural details of the active site of novel FP's that are not provided by X-ray crystallography, in particular, protonation states and hydrogen-bonded networks.
- Study the mechanisms fluorescence quenching (internal conversion pathways).
- Analyze the effects of mutations and structural features on the optical properties and on the ground- and excited-state potential energy surfaces.
- Study the excited-state proton transfer, which is competing with photoisomerization, determine proton-transfer pathways, barriers, and their modulation by the local (structural) environment, as well as by the protein electrostatics.
Develop atomistic models of the complex photophysical phenomena observed in native and engineered FP’s and to identify the structural factors that are responsible for the large differences in absorption and emission wave length, absorptivity, inhomogenuous line broadening, pH sensitivity, fluorescence lifetime and QY.
Four main aspects shall be addressed:
- Advanced Methods and Applications in Quantum Chemistry (Stuttgart, Germany, March 26-30, 2012) --->
- Spanish Government FPI grant
Dr. Marius Wanko
Prof. Angel Rubio